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Technical Support

Frequently Asked Questions

We have listed the most commonly asked questions below.  If your question and answer are not listed, please e-mail techsupt@brendan.com with your question.  Our technical support staff will address your question and e-mail the answer to you.
 
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How do I start using StatLIA?
 
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Can I customize the assay report?
 
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What does the "In Use" message mean?
 
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Can I rearrange the order of the assays in the Assay Table?
 
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What does a status of W-Comput mean?
 
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Can I collect the data without computing the assay?
 
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Can I compute an assay from data that's already been collected?
 
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Can I edit the data and re-compute the assay?
 
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How do I control which workstation is computing the assay?
 
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Can I delete an assay from the reference or current set?
 
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Can I set the maximum number of reference assays to a number less than 30?
 
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Can I export data to an external database or spreadsheet program?
 
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Can I import worklists from an external database or spreadsheet program?
 
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What is the adjusted response?
 
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What is the normalized response?
 
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What is CQA?
 
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Does StatLIA create an audit trail as I use the program?
 
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What are the Reference and Current sets?
 
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Can I retrieve an old assay that does not appear in the reference or current set?
 
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What does Available and Unavailable mean?
 
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Can I send a start command to an instrument using StatLIA?
 
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Do I have to do an Assay Run Setup each time in order to compute an assay?

How do I start using StatLIA?

 
1
Install the instrument driver by clicking on the Laboratory icon on the main screen.  Connect the cable from the instrument to the computer (direct connection) or to one of the ports on the BayTech buffer box (multiple instrument connection).
 
2
Setup a Test Method by clicking on the Test Methods icon on the main screen.  The test method defines the protocol that is used for each assay.  For example, the number and concentration of standards, the number of controls and the number of replicates of each are defined in Test Method Setup.  The Test Method Setup is performed only once for each analyte that is run in the laboratory.
 
3
Setup an Assay Run by clicking on the Setup icon on the main screen.  The Assay Run is the group of immunoassay samples that are run daily.
 
4

Compute the Assay Run.  StatLIA continuously collects data from the instruments and computes the assay if the collecting cycle is activated.   To do this, toggle the button on the main screen from Not Collecting to Collecting. The data can also be computed manually using the Compute button on the main screen.

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Can I customize the assay report?

  Yes. StatLIA's Assay Report can include up to eight sections.  The Unknown Results and Assay Info are the only two sections included with every report.
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What does the "In Use" message mean?

  StatLIA will print (In Use) next to a test method name if the file is open on another workstation or if the file was not closed properly.  Make sure that the test method file is not being used on another workstation before attempting to reset or close the file.  To reset a file, click on the Instrument icon on the main screen.  Select Reset, select the test and click on Select at the bottom of the dialog box.
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Can I rearrange the order of the assays in the Assay Table?

  Yes.  Assays can be re-arranged by clicking on the assay then clicking on the new position.  This is only needed for assays that do not use the Batch Name option.  This option allows you to enter a character string (Batch Name) when setting up the assay.  The raw data file is searched and if this character string is not found, the assay is not computed.  For this reason only assays that do not use the batch name option are computed in the order that they appear in the Assay Table.
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What does a status of W-Comput mean?

  The status of W-Comput means that the data has been collected from the instrument and assigned to the Assay Setup and is waiting for the next cycle to compute.  Once computed, the assay is added to the database and removed from the Assay Table.
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Can I collect the data without computing the assay?

  Yes.  If the status of the assay is set to Hold the data is collected and matched to the Assay Setup but not computed.   To compute the assay, click on the Compute icon.  Select Compute, select the assay and click on Compute at the bottom of the dialog box.
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Can I compute an assay from data that's already been collected?

Yes.  All data that has been collected from instruments are stored in raw data files.  To compute an assay from a raw data file:
 
1
Setup the assay as usual.
 
2
Click on the Instrument icon at the bottom of the main screen.
 
3
Select Raw Data and Raw Data again at the dialog boxes.
 
4
The Raw Data File Selection screen appears.  Click on the Detector Name.
 
5
Click on the appropriate Julian Day folder.   The Julian Day is the two digit year code followed by the number of days since January 1st.  Today's Julian day is always the one on the bottom of the list.
 
6

Click on the appropriate raw data file.  These files are named with the time of day that they were collected.

 
7
The file can be viewed to confirm that this is the correct file by clicking on View at the bottom of the screen.
 
8
Click on Compute at the bottom of the screen.
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Can I edit the data and re-compute the assay?

  Yes.  The data can be edited (if security is turned off) or set to Ignore (if security is turned on).  If the security log is turned on, any changes to the data are recorded.  More information about editing data can be found in the tutorial brief Recomputing an Assay and Editing the Standard Curve.
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How do I control which workstation is computing the assay?

  StatLIA will compute the next assay on the Available workstation with the highest workstation number.  If you want the assay computed on another workstation, set the status to Hold, just before saving the Assay Setup.  After the data is collected, the status changes to W-Hold and can be computed by using the Compute button on the main screen from any workstation.
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Can I delete an assay from the reference or current set?

  Yes.  Any assay can be deleted, copied or moved from the reference or current set.  More information can be found in the Operator's Guide, Deleting Copying or Moving Assay Runs.
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Can I set the maximum number of reference assays to a number less than 30?

  Yes.  If you want to stop the accumulation of reference assays at a number less than 30, enter 30 as the Reference Counter number after you've reached the desired number of assays in the reference set.   In this way, StatLIA will not add any more assays to the reference set.
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Can I export data to an external database or spreadsheet program?

  Yes.  All of the raw data and computed values can be exported as ASCII text files to an external database or spreadsheet format.  Four delimitation formats are available: Fixed, Comma, Tab and Encapsulated.  More information is available in the Operator's Guide, Exporting Test Data.
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Can I import worklists from an external database or spreadsheet program?

  Yes.  Worklists containing the identification and demographic information about the patients or unknown specimens for an assay can be imported from external database or spreadsheet programs, such as Access or Excel.   These files are saved as ASCII text files in the STATLIA\LIMIN folder.  Available formats are Fixed, Comma, Tab and ASTM.  More information about the LIM file specifications is available in the Specifications document.
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What is the adjusted response?

  The adjusted response is the raw response (e.g., CPM, ABS, RLU), adjusted to remove assay to assay signal variation.  The adjusted (and normalized) responses are an integral part of StatLIA's troubleshooting process.  If a tracer activity is run, StatLIA adjusts all raw responses to the activity that would have been observed if a set tracer activity was added.  If no tracer activity is run, the adjusted response is the same as the raw response.  More information about the adjusted response can be found in the glossary.
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What is the normalized response?

  The normalized response is the adjusted response (e.g., CPM, ABS, RLU), divided by a baseline standard or standard adjusted response, (such as the B0 or Standard 6).  The adjusted and normalized responses are an integral part of the StatLIA troubleshooting process.  More information about the normalized response can be found in the glossary.
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What is CQA?

  CQA stands for Component Quality Analysis.  StatLIA evaluates the integrity of the assay components (e.g., tracer, antibody, buffer, incubation conditions, standards, control specimens, etc.) by statistically evaluating the immunoassay parameters (e.g., baseline standard and standard responses, control specimen responses, minimum and maximum detectable concentrations, etc.).  CQA refers to a process whereby all of the immunoassay parameters are statistically compared to their corresponding values from a stable set of reference assays.  Components behaving differently from these validated reference assays are identified by the statistically flagged parameters.
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Does StatLIA create an audit trail as I use the program?

  Yes.  StatLIA has 28 checkpoints throughout the program that are restricted by a three level password system.  If security is turned on for that checkpoint, you will get a password box when you enter.  With or without the password security turned on, you can log any activity for that checkpoint in the Security Log.  More information about Security can be found the the Laboratory Setup section of the Operator's Guide.
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What are the Reference and Current sets?

  The reference assay set is typically the first thirty assays that are run.  The current assay set is the last thirty assays that have been run.  The reference assays remain fixed (unless changed by the laboratory) but the current set continually deletes the oldest assay when a new assay is processed.   StatLIA's troubleshooting process statistically compares the computed assay parameters to the reference set parameters to detect changes in the performance of any reagents or incubation steps.  More information about the reference and current sets can be found in the Glossary.
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Can I retrieve an old assay that does not appear in the reference or current set?

  Yes. All assays in the reference or current assay set can be archived and subsequently retrieved at any time.   New assays are automatically archived every thirty assays  if the archive switch is set in Test Method Setup/Evaluation Specification. 
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What does Available and Unavailable mean?

  The Available/Unavailable button allows you to choose if you want that StatLIA workstation to compute new assays automatically (Available) or not compute new assays automatically (Unavailable).  If Available is chosen, the collecting workstation distributes assays to the next available workstation (with the highest workstation number) for automatic computation.
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Can I send a start command to an instrument using StatLIA?

Yes.  The Terminal program in StatLIA allows you to send line commands to any instruments that are connected to either the communications port on the computer or the BayTech buffer box.
 
1
From the main screen click on the Instrument icon.
 
2
Click on the Instrument button.
 
3
Click on the Terminal button.
 
4
Select the Instrument and click on Select at the bottom of the selection box.
 
5
At the Terminal screen, type in a command and hit the Enter key.
 
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To receive the response from the instrument hit the Enter key again at the next question mark prompt.

 
7
Repeat steps 5 and 6 until the terminal session is finished.
 
8
Type Exit to exit the Terminal program.
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Do I have to do an Assay Run Setup each time in order to compute an assay?

  Normally.  However, StatLIA offers an "Autoassay" option which allows you to create a file (with the necessary setup information) from another application and save it in the STATLIA\RAWASSAY folder.  StatLIA examines that folder periodically and, if any files are there, automatically creates the setup file and (1) computes the assay if the raw responses are in the file or (2) waits for the data to be collected from the instrument.  This is useful for laboratories introducing StatLIA in the middle of on-going studies by allowing them to process their previously computed assays again in StatLIA in one operation without having to do a separate assay run setup for each assay.  This is also a helpful capability for Instrument Manufacturers or LIM systems that have the information is already entered by eliminating the need to enter the same information twice.  More information can be found in the Specifications.
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