Enzyme Multiplied Immunoassay Technique

Enzyme multiplied immunoassay technique (EMIT) assays are homogenous competitive binding enzyme immunoassays in which a low molecular weight ligand is attached to an enzyme. Enzyme activity is inhibited when the enzyme labeled conjugate is bound to a binder. Addition of unlabeled ligand analyte results in a competition for binding sites on the binder and increasing amounts of free enzyme conjugate as the concentration of unlabeled ligand increases. The standard curve of an EMIT assay has a positive slope and is often asymmetrical.