Immunoassay
An immunoassay is a ligand-binding as ay that uses an antibody and the antibody’s corresponding antigen as the binder and ligand. The term immunoassay is often expanded to include any procedure in which the quantitation of an analyte depends on the progressive saturation of a specific binder or ligand, either of which may be the analyte, and the subsequent determination of the analyte’s distribution between the bound and free fractions. This includes competitive protein binding assays, where the binder is a naturally occurring binding protein, and receptor assays, where the binder is a naturally occurring cell receptor.
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