Nonspecific Binding

Nonspecific binding (NSB) is the measurement of label or labeled conjugate present in the bound fraction of a ligand-binder reaction that occurred from nonimmune-specific means. Nonspecific binding is the only type of binding found in competitive binding immunoassay samples having no binder present, and in sandwich binding immunoassay samples (zero blank) having no ligand present. Nonspecific binding samples as a baseline Bmin can be used to represent infinitely high standard concentrations (competitive binding) or infinitely low standard concentrations (sandwich binding).