Edge Effect And Randomization Pipetting
It has been demonstrated that raw signal measurements are not uniform in all regions of the plate. These small positional effects can disproportionately affect assay results and the reliability of the data. You can reduce the positional bias of your potency assays by creating placement templates in STATLIA MATRIX. One common strategy is to avoid the edge effect by not using outer wells of the plate. Another strategy is to use a randomization scheme that completely randomizes the placement of all dilutional replicates.